Ionic-liquid-assisted desorption of DNA from polyamidoamine-grafted silica nanoparticles surface by a low-salt
Nurgul Latep1, Xiao Liang, Weidong Qin
1Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry, Beijing Normal University, Beijing, China.
Abstract:
We studied the influences of imidazolium-based ionic liquids as additives in low ionic strength phosphate solution on releasing DNA from polyamidoamine dendrimer-grafted silica nanoparticle surfaces. The effects of the side-chain length of the imidazolium group, the anion and the concentration of the ionic liquid, the generation of the dendrimer, and the pH and the concentration of the release solution were investigated. It was found that addition of 4 mM 1-hexyl-3-methylimidazolium bromide to 5 mM phosphate at pH 11 could markedly promote the desorption of DNA fragments, with a desorption efficiency of 99.0%. Compared with the conventional strategies employing high-salt solutions or elevated temperature for acceptable recoveries, the method described here enabled quick release of DNA fragments that permitted direct, accurate analysis, and further treatment without desalting.
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