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Hemolysis-free blood plasma separation.

Jun Ho Son1, Sang Hun Lee, Soongweon Hong

  • 1Department of Bioengineering, Department of Electrical Engineering and Computer Sciences, Biophysics Program, Berkeley Sensor and Actuator Center, University of California, Berkeley, CA 94720, USA. lplee@berkeley.edu.

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This study introduces a novel hemolysis-free microfluidic device for blood plasma separation. The innovative filter-in-top design significantly reduces red blood cell lysis, improving diagnostic accuracy for point-of-care applications.

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Area of Science:

  • Biomedical Engineering
  • Clinical Diagnostics
  • Microfluidics

Background:

  • Hemolysis, or red blood cell rupture, releases inhibitors that compromise diagnostic tests.
  • Current plasma separation methods face challenges with clogging and hemolysis, hindering point-of-care diagnostics.
  • A reliable, hemolysis-free method is crucial for advancing microfluidic diagnostic devices.

Purpose of the Study:

  • To develop and validate a hemolysis-free microfluidic platform for efficient blood plasma separation.
  • To overcome limitations of conventional methods in preventing red blood cell lysis during plasma processing.
  • To enable accurate and sensitive downstream diagnostic assays on microfluidic devices.

Main Methods:

  • A novel microfluidic device featuring a filter-in-top configuration with a vertical up-flow channel.
  • Utilized gravity-assisted cell sedimentation to minimize red blood cell clogging.
  • Evaluated plasma separation efficiency, hemolysis reduction, and target molecule recovery.

Main Results:

  • Achieved a ~4-fold increase in separated plasma volume compared to filter-in-bottom configurations.
  • Reduced hemoglobin concentration in plasma by ~90%, indicating significant hemolysis prevention.
  • Demonstrated comparable recovery of proteins (~90%) and nucleic acids (~100%) to off-chip methods.

Conclusions:

  • The developed hemolysis-free microfluidic platform offers a simple, robust solution for blood plasma separation.
  • This technology significantly enhances plasma purity and diagnostic reliability for microfluidic point-of-care devices.
  • Enables seamless integration with downstream modules for complete sample-to-answer diagnostic systems.