Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Microbial Growth Measurement: Direct Methods

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Blinded 2-Year Longitudinal Evaluation of SARS-CoV-2 Antigenemia in Long COVID.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026

Same author

Digital seed amplification assay for TDP-43 aggregate quantification in CSF.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026

Same author

Differences in SARS-CoV-2 Antigen Persistence in Individuals With Systemic Autoimmune Rheumatic Diseases Compared to the General Population: A RECOVER-Adult Cohort Study.

Arthritis & rheumatology (Hoboken, N.J.)·2026

Same author

High-throughput single-vesicle imaging platform for direct extracellular vesicle profiling of human plasma.

Nature communications·2026

Same author

Endovascular profiles linked to neutrophil activation in children and young adults with long COVID.

Pediatric research·2026

Same author

Attomolar fecal cytokine profiling reveals gut immune dynamics and disease states.

bioRxiv : the preprint server for biology·2026

Search research articles

Related Experiment Video

Updated: Apr 29, 2026

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Published on: November 4, 2009

Protein measurements in microwells.

¹Tufts University, Department of Chemistry, Medford, MA 02155, USA. david.walt@tufts.edu.

|May 15, 2014

Summary

This summary is machine-generated.

Ultra-sensitive protein measurements using microwell arrays can aid early disease diagnosis and single-cell analysis. This technology offers high local concentrations for improved detection limits in protein assays.

More Related Videos

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Published on: July 27, 2022

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Published on: November 18, 2013

Related Experiment Videos

Last Updated: Apr 29, 2026

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Microvolume Protein Concentration Determination using the NanoDrop 2000c Spectrophotometer

Published on: November 4, 2009

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Enhanced Reproducibility and Precision of High-Throughput Quantification of Bacterial Growth Data Using a Microplate Reader

Published on: July 27, 2022

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates

Published on: November 18, 2013

Area of Science:

Biochemistry
Clinical Diagnostics
Cell Biology

Background:

Protein measurements are vital across scientific disciplines.
Current protein assay methods have detection limits hindering early disease diagnosis.
Single-cell analysis is growing, but protein measurement methods are less developed than genetic targets.

Purpose of the Study:

To review the current state of microwell arrays for protein measurements.
To identify challenges and opportunities for future microwell applications in protein analysis.

Main Methods:

Utilizing microwells and microwell arrays to confine molecules and cells.
Achieving high local concentrations within small volumes for enhanced detection.

Main Results:

Microwell arrays demonstrate potential for ultra-sensitive protein measurements.
Confined volumes in microwells increase local analyte concentrations.

Conclusions:

Microwell arrays are promising platforms for advancing protein measurement sensitivity.
Future developments in microwell technology could overcome current limitations in protein detection and single-cell analysis.