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Yeast As a Chassis for Developing Functional Assays to Study Human P53
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Does not hUTP14a promoter form a regulation feedback loop with P53?

Jingyi Zhang1, Yafei Guo1, Xiaojuan Du1

  • 11 Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Hepatic, Biliary & Pancreatic Surgery I, Peking University Cancer Hospital & Institute, Beijing 100142, China ; 2 Department of Cell Biology, Peking University Health Science Center, Beijing 100191, China.

Chinese Journal of Cancer Research = Chung-Kuo Yen Cheng Yen Chiu
|May 15, 2014
PubMed
Summary

Human UTP14A (hUTP14a) binds and degrades P53, but its own transcription is not regulated by P53. This study identified the hUTP14a promoter region, revealing no feedback loop between hUTP14a and P53.

Keywords:
P53hUTP14aluciferase activitypromotertranscription factor

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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cancer Research

Background:

  • The protein hUTP14a interacts with and promotes the degradation of P53.
  • It is unknown whether hUTP14a is a downstream target gene of P53.

Purpose of the Study:

  • To identify the promoter region of the hUTP14a gene.
  • To investigate the regulatory relationship between hUTP14a transcription and P53.

Main Methods:

  • Cloning of the hUTP14a promoter into a luciferase reporter plasmid.
  • Transfection into 293T cells and luciferase activity assays.
  • Bioinformatic identification of transcription factors and P53 co-transfection experiments.
  • Determination of the minimal promoter region using deletion constructs.

Main Results:

  • The hUTP14a promoter construct exhibited significant activity.
  • P53 did not regulate the transcription of hUTP14a.
  • The minimal promoter region of hUTP14a was mapped to the sequence between -203 and -100 relative to the transcription start site.

Conclusions:

  • hUTP14a transcription is not directly regulated by P53.
  • Unlike other P53-interacting proteins, hUTP14a does not form a transcriptional feedback loop with P53.