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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
Published on: August 31, 2019
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Liposomes physically coated with peptides: preparation and characterization.
Cuicui Su1, Yuqiong Xia, Jianbo Sun
1Beijing National Laboratory for Molecular Sciences and the Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.
Langmuir : the ACS Journal of Surfaces and Colloids
|May 16, 2014
Summary
Peptide coating offers an efficient method for creating functional drug delivery liposomes. This study demonstrates peptide-modified liposomes exhibit controlled release and dual pH/temperature responsiveness.
Area of Science:
- Biomaterials Science
- Drug Delivery Systems
- Nanotechnology
Background:
- Liposomes are versatile drug delivery vehicles.
- Surface modification of liposomes enhances their functionality.
- Peptide coating presents an economical and efficient strategy for liposome functionalization.
Purpose of the Study:
- To design and synthesize novel peptides for liposome coating.
- To investigate the coating efficiency and stability of peptide-functionalized liposomes.
- To evaluate the responsive behavior and drug release characteristics of modified liposomes.
Main Methods:
- Synthesis of three distinct peptides (W2K3, W2R3, W2G3).
- Coating of negatively charged liposomes with synthesized peptides.
- Characterization of peptide-liposome interactions using electrostatic principles.
- Assessment of liposome stability, pH/temperature responsiveness, and drug release kinetics.
Main Results:
- Peptide coating is primarily driven by electrostatic interactions between cationic peptides and anionic lipids.
- W2R3 peptide showed higher coating efficiency compared to W2K3, but decreased liposome stability due to arginine's guanidinium group.
- Peptide-coated liposomes exhibited pH-responsive behavior and controlled content release without significant changes in phase transition temperature.
Conclusions:
- Peptide coating is a viable method for creating functionalized liposomes.
- The choice of peptide influences coating efficiency and liposome stability.
- Peptide-modified liposomes demonstrate promising pH/temperature dual-responsive drug release capabilities.
