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Updated: Aug 10, 2026

Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Published on: December 27, 2016
One- and two-dimensional 1H NMR study of the substance P fragment ARG-PRO-Lys-Pro
A Otter1, G Kotovych, J M Stewart
1Department of Chemistry, University of Alberta, Edmonton, Canada.
Abstract:
The Substance P fragment Arg1-Pro2-Lys3-Pro4 (SP1-4) has been extensively investigated by means of proton nuclear magnetic resonance at 400 MHz. The combined application of different 2D techniques and a comparison of SP1-4 with its derivative SP1-4-amide allowed the complete and unambiguous assignment of the proton NMR spectrum. Conformational data obtained from the different NMR parameters are compared with theoretical calculations. The results suggest that SP1-4 exists, at the chosen experimental conditions, as a stretched molecule.
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