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Updated: Apr 29, 2026

Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
Published on: June 21, 2022
Intratumor heterogeneity and its impact on drug distribution and sensitivity
I Fuso Nerini1, L Morosi1, M Zucchetti1
1IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri," Laboratory of Cancer Pharmacology, Department of Oncology, Milan, Italy.
Abstract:
We provide an overview of the available information on the distribution of chemotherapeutics in human tumors, highlighting the progress made to assess the heterogeneity of drug concentrations in relation to the complex neoplastic tissue using novel analytical methods, e.g., mass spectrometry imaging. The increase in interstitial fluid pressure due to abnormal vascularization and stiffness of tumor stroma explains the variable and heterogeneous drug concentrations. Therapeutic strategies to enhance tumor drug distribution, thus possibly increasing efficacy, are discussed.
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