In vitro interaction of bleomycin with collagen--equilibrium dialysis technique

In an earlier communication, we have presented the results of our study on in vitro interaction of bleomycin with collagens of sepia, fish and rat skins using spectrophotometry. Since the spectrophotometric method does not involve physical separation of the bound and unbound portion of the drug, it was considered worthwhile to test the validity of the use of spectral data by equilibrium dialysis. In the present communication, the values of binding between bleomycin and soluble collagens representing vertebrate, lower vertebrate and invertebrate determined independently at pH 7.4 and 3.0 are presented.