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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Enzymatic cocktails produced by Fusarium graminearum under submerged fermentation using different lignocellulosic
Philippe Debeire1, Francois Delalande, Olivier Habrylo
1Institut de Chimie et Procédés pour l'Énergie, l'Environnement et la Santé (UMR 7515 Strasbourg University/CNRS), ECPM, Strasbourg Cedex 2, France.
Abstract:
Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall-degrading enzymes (CWDE) proportion varied greatly from 20% to 69%. Only one of the 57 CWDEs detected in this study was common to the five proteomes. In contrast, 35 CWDEs were specific to one proteome only. The polysaccharide-degradation activities were different depending on the cocktail and the polysaccharide used. F. graminearum strongly modifies the enzymatic cocktail it secretes as a function of the biomass used for growth.
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