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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
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Engineering the lactococcal mevalonate pathway for increased sesquiterpene production
Adelene A Song1, Janna Ong Abdullah, Mohd P Abdullah
1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor Darul Ehsan, Malaysia.
FEMS Microbiology Letters
|May 16, 2014
Summary
Lactococcus lactis, a food-grade bacterium, can be engineered for isoprenoid production. Co-expressing mvk and mvaA genes in L. lactis doubled the production of β-sesquiphellandrene, enhancing its potential as a host.
Area of Science:
- Metabolic engineering
- Synthetic biology
- Microbial biotechnology
Background:
- Isoprenoids are vital secondary metabolites with renewed interest due to genetic engineering.
- Escherichia coli and yeasts are common hosts, utilizing MEP and mevalonate pathways, respectively.
- Lactococcus lactis, a food-grade Gram-positive bacterium, utilizes the mevalonate pathway, making it an attractive alternative host.
Purpose of the Study:
- To engineer Lactococcus lactis as an efficient host for heterologous isoprenoid production.
- To analyze the gene expression of the lactococcal mevalonate pathway.
- To increase β-sesquiphellandrene production in L. lactis.
Main Methods:
- RT-qPCR analysis of the lactococcal mevalonate pathway gene expression.
- Heterologous expression of plant sesquiterpene synthases in L. lactis.
- Overexpression of mvk and co-expression of mvk with mvaA genes.
Main Results:
- Gene expression of the lactococcal mevalonate pathway was analyzed.
- L. lactis successfully produced sesquiterpenes via heterologous expression.
- Co-expression of mvk and mvaA genes doubled β-sesquiphellandrene production.
Conclusions:
- Lactococcus lactis is a promising host for isoprenoid production.
- Engineering the mevalonate pathway enhances isoprenoid yield in L. lactis.
- Co-expression of mvk and mvaA represents an effective strategy for boosting β-sesquiphellandrene biosynthesis.
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