Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kaempferol Attenuates Spaceflight-Associated Knee Cartilage Degradation by Targeting NOX4-Mediated Mitochondrial Dysfunction.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Repurposing Metformin to Promote Fracture Callus Maturation via AMPK-Driven Metabolic Activation.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same author

Holistic genome assembly and analysis of the <i>Tremella fuciformis</i> interaction community uncovers intergenomic insights beyond dual genomes.

IMA fungus·2026
Same author

A translational approach to airway reconstruction leveraging decellularized meniscus and cartilage progenitor cells.

Nature communications·2026
Same author

Li<sup>+</sup>-Mediated Topological Regulation of Aluminosilicate Glass Ceramics: Near-Full Crystallinity for Multifunctional Optoelectronic Applications.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Integrated transcriptomic analysis identifies tsRNA-mRNA regulatory axis in asthma pathogenesis.

The World Allergy Organization journal·2026

Related Experiment Video

Updated: Apr 29, 2026

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs
12:44

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Published on: January 27, 2023

3.6K

Stem cell-based microphysiological osteochondral system to model tissue response to interleukin-1β.

Hang Lin1, Thomas P Lozito, Peter G Alexander

  • 1Center for Cellular and Molecular Engineering, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine , Pittsburgh, Pennsylvania 15219, United States.

Molecular Pharmaceutics
|May 17, 2014
PubMed
Summary

This study developed a novel microfluidic bioreactor to engineer osteochondral tissue, revealing communication between bone and cartilage that influences osteoarthritis progression and drug testing.

More Related Videos

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
07:10

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Published on: November 3, 2019

8.8K
A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
08:28

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

Published on: May 21, 2020

6.7K

Related Experiment Videos

Last Updated: Apr 29, 2026

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs
12:44

Creation of a Knee Joint-on-a-Chip for Modeling Joint Diseases and Testing Drugs

Published on: January 27, 2023

3.6K
An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants
07:10

An Ex Vivo Tissue Culture Model of Cartilage Remodeling in Bovine Knee Explants

Published on: November 3, 2019

8.8K
A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
08:28

A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling

Published on: May 21, 2020

6.7K

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Osteoarthritis Research

Background:

  • Osteoarthritis (OA) is a degenerative joint disease affecting bone and cartilage.
  • Understanding OA pathogenesis and developing disease-modifying drugs (DMOADs) requires physiological models.
  • Current models lack the complexity to replicate osteochondral interactions.

Purpose of the Study:

  • To engineer functional osteochondral tissue in vitro.
  • To investigate the pathogenic mechanisms of OA using a novel bioreactor system.
  • To establish a platform for testing potential DMOADs.

Main Methods:

  • Fabrication of a multichamber microfluidic bioreactor.
  • In situ fabrication and culture of human bone marrow stem cell (hBMSC)-derived osteochondral constructs.
  • Induction of chondrogenic and osteogenic differentiation with tissue-specific media.
  • Application of interleukin-1β (IL-1β) to assess degradative responses.

Main Results:

  • Successful engineering of osteochondral tissue with distinct chondral and osseous layers.
  • Observed tissue-specific gene expression, matrix production, and a tidemark interface.
  • IL-1β induced significant degradative responses in both local and opposing tissues.
  • Osseous IL-1β application caused greater chondral degradation, indicating cross-talk.

Conclusions:

  • The developed microtissue culture system effectively mimics osteochondral tissue physiology.
  • Demonstrated active biochemical communication between bone and cartilage layers in OA.
  • The system serves as a valuable platform for OA research and DMOAD screening.