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The Significance of Membrane Transport01:44

The Significance of Membrane Transport

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The transport of solutes across the cell membrane is essential for metabolic processes, like maintaining cell size and volume, generating the action potential, exchanging nutrients and gases, etc. Membrane transport can be either passive or active. It can be simple diffusion, facilitated, or mediated transport aided by transport proteins such as transporters and channels.
Transporters facilitate either an active or passive movement of solutes. They can allow a single-molecule transport down its...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

Updated: Apr 29, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
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Quantitative targeted proteomics for membrane transporter proteins: method and application.

Xi Qiu1, Hui Zhang, Yurong Lai

  • 1Pharmaceutical Candidate Optimization, Bristol-Myers Squibb, Princeton, New Jersey, 08543, USA.

The AAPS Journal
|May 17, 2014
PubMed
Summary

Targeted proteomics accurately quantifies low-abundance proteins, crucial for understanding drug metabolism and excretion (ADME). This method aids in reliable in vitro-in vivo extrapolations (IVIVE) for better pharmacokinetic predictions.

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Area of Science:

  • Proteomics
  • Biopharmaceutical Research
  • Pharmacokinetics

Background:

  • Global proteomics identifies new proteins but struggles with low-abundance functional proteins.
  • Drug absorption, distribution, metabolism, and excretion (ADME) proteins are critical in biopharmaceutical research.
  • Variations in ADME protein expression complicate in vitro-in vivo and in vivo-in vivo extrapolations (IVIVE).

Purpose of the Study:

  • To review methodologies for targeted proteomics.
  • To summarize recent applications of targeted proteomics in biopharmaceutical research.
  • To highlight the importance of accurate ADME protein quantification for IVIVE.

Main Methods:

  • Mass spectrometry-based targeted quantification.
  • Overview of relevant proteomics methodologies.
  • Review of recent applications focusing on membrane transporters.

Main Results:

  • Targeted proteomics complements untargeted methods for specific protein measurement.
  • Accurate quantification of ADME proteins is fundamental for reliable IVIVE.
  • Mass spectrometry enables precise measurement of low-abundance functional proteins.

Conclusions:

  • Targeted proteomics is essential for measuring key ADME proteins.
  • Accurate quantification of ADME proteins using targeted proteomics improves pharmacokinetic predictions.
  • This approach is vital for advancing biopharmaceutical research and drug development.