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Opening up the optical imaging window using nano-luciferin.

Apurva R Patel1, Ed Lim, Kevin P Francis

  • 1College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, Florida, 32307, USA.

Pharmaceutical Research
|May 17, 2014
PubMed
Summary

Developed novel nanoparticles for D-luciferin (Nano-Luc) and dual-functional DiR/luciferin (Nano-LucDiR) enabling prolonged in-vivo tumor imaging and tracking. These formulations enhance luciferin circulation, improving tumor visualization for over 24 hours.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Medical Imaging

Background:

  • In-vivo imaging is crucial for tumor detection and monitoring.
  • Current imaging agents may have limitations in circulation time and signal detection.
  • Nanoparticle-based drug delivery systems offer potential for enhanced imaging capabilities.

Purpose of the Study:

  • To formulate D-luciferin (Nano-Luc) and dual-functional DiR/luciferin (Nano-LucDiR) nanoparticles.
  • To evaluate their efficacy for in-vivo tumor imaging and nanoparticle tracking.
  • To optimize nanoparticle formulation using design of experiments.

Main Methods:

  • Nanoparticles (Nano-Luc, Nano-DiR, Nano-LucDiR) were prepared and characterized for physical properties, loading, and release.
  • Response Surface Methodology (RSM) and Design of Experiments (DOE) were used for optimization.
  • In-vivo imaging and tracking were performed in various mouse tumor models using IVIS® Spectrum-CT.

Main Results:

  • Nano-Luc and Nano-LucDiR nanoparticles were successfully formulated with sizes <200 nm.
  • Optimized Nano-Luc showed high loading (5.0 mg/ml) and encapsulation (99%) efficiencies with controlled release.
  • Nano-Luc and Nano-LucDiR significantly enhanced luciferin plasma half-life, enabling tumor imaging for over 24 hours; Nano-LucDiR allowed simultaneous bioluminescent and fluorescent imaging.

Conclusions:

  • Nano-Luc and Nano-LucDiR nanoparticles facilitate prolonged and reproducible in-vivo tumor imaging.
  • These formulations are particularly effective for multimodality 3D imaging.
  • The developed nanoparticles show promise for advanced tumor visualization and tracking.