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Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
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Expression and function of endothelial selectins during human development.

Kaisa Auvinen1, Sirpa Jalkanen, Marko Salmi

  • 1MediCity Research Laboratory, University of Turku, Turku, Finland; National Institute for Health and Welfare Turku, Turku, Finland.

Immunology
|May 17, 2014
PubMed
Summary
This summary is machine-generated.

Selectins are crucial for immune cell movement. This study reveals that selectins and their ligands are functional in human fetuses, enabling mononuclear cell migration and immune responses before birth.

Keywords:
adhesion moleculescell recruitment/emigrationendotheliumontogenyselectins

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Area of Science:

  • Immunology
  • Developmental Biology
  • Cell Biology

Background:

  • Leukocyte trafficking is essential for immune defense in adults, mediated by selectins and their ligands.
  • The role of selectins in fetal mononuclear cell migration during human development is currently unknown.

Purpose of the Study:

  • To investigate the function of endothelial E- and P-selectins and their counter-receptors during human development (ontogeny).

Main Methods:

  • Immunohistochemical staining to determine the expression patterns of P-selectin and E-selectin.
  • In vitro flow chamber assays using real-time imaging to analyze mononuclear leukocyte interactions with endothelium.

Main Results:

  • P-selectin expression was observed in megakaryocytes (from gestational week 7) and endothelial cells (from week 11).
  • Endothelial E-selectin expression was detected later, at week 32.
  • Cord blood mononuclear leukocytes utilized E-, P-, and L-selectin, along with PSGL-1, for rolling and adhesion to the endothelium under physiological shear stress.

Conclusions:

  • Selectins are synthesized and become functional in humans prior to birth.
  • These selectin-mediated interactions have the potential to facilitate mononuclear cell emigration and initiate inflammatory responses during fetal development.