[Factors influencing the determination of myoglobin in human blood serum using an immunoenzyme method]

Some factors influencing the preservation of myoglobin titre in human blood serum, measured by enzyme immunoassay (EIA), were under study. The optimal storage conditions were selected: +4 degrees C in the presence of 0.005 M ethylenediamine tetraacetic acid, 0.001 M phenylmethylsulfonylfluoride, and 0.01% merthiolate. The authors suppose that myoglobin titre reduces over the course of serum storage because of myoglobin molecule proteolytic degradation and conformation changes in aggregate transition. Serum dilution made to choose the optimal signal/background ratio is another essential factor in EIA measurement of myoglobin concentrations.