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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Updated: Apr 29, 2026

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
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Genotyping-by-Sequencing in Plants.

Stéphane Deschamps1, Victor Llaca2, Gregory D May3

  • 1DuPont Agricultural Biotechnology, Experimental Station, PO Box 80353, 200 Powder Mill Road, Wilmington, DE 19880-0353, USA. stephane.deschamps@usa.dupont.com.

Biology
|May 17, 2014
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Summary
This summary is machine-generated.

Next-generation sequencing enables rapid, genome-wide detection of Single Nucleotide Polymorphisms (SNPs) in plants. Genotyping-by-sequencing (GBS) bypasses marker development for efficient large-scale genetic analysis.

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Area of Science:

  • Plant genomics
  • Molecular biology
  • Bioinformatics

Background:

  • Next-generation sequencing (NGS) technologies have revolutionized plant genetic research.
  • Cost reductions and increased throughput enable large-scale population studies.

Purpose of the Study:

  • To review the current applications of genotyping-by-sequencing (GBS) in plants.
  • To highlight the potential of GBS for genome-wide genotyping.

Main Methods:

  • Genotyping-by-sequencing (GBS) utilizes NGS to detect and score Single Nucleotide Polymorphisms (SNPs).
  • This method bypasses traditional marker assay development stages.

Main Results:

  • GBS allows for rapid, genome-wide SNP detection in diverse plant species.
  • It facilitates the mapping and characterization of traits in large populations.

Conclusions:

  • GBS is a powerful and efficient tool for plant genome-wide genotyping.
  • Its application promises to accelerate genetic improvement and trait discovery in plants.