Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.9K
RNA-seq03:21

RNA-seq

9.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circtools 2.0: a comprehensive framework for enhanced circular RNA bioinformatics.

BMC bioinformatics·2026
Same author

The SURROGATOR Framework for Context-Aware Surrogation of Privacy Sensitive Information in Medical Text.

Studies in health technology and informatics·2026
Same author

RBM20 isoform regulation by independent transcription start sites adapts alternative splicing in development and disease.

Nature communications·2026
Same author

CAMK2D causes heart failure in mice with RBM20 cardiomyopathy.

Nature cardiovascular research·2026
Same author

Enhancing KLF15 activity in cardiomyocytes: a novel approach to prevent pathological reprogramming and fibrosis via nuclease-deficient dCas9VPR.

Signal transduction and targeted therapy·2026
Same author

The two-step purification method ViREn identifies a single NSUN6-mediated 5-methylcytosine modification promoting dengue virus RNA genome turnover.

Nucleic acids research·2026
Same journal

Spatial Heterogeneity of Phytoplankton Taxa and Functional Groups Under Multidimensional Environmental Factors in Karst Urban Rivers.

Biology·2026
Same journal

Paleopathology of a Lower Miocene Carettochelyid Turtle from the Moghra Formation, Egypt.

Biology·2026
Same journal

Effects of Type I Diabetes Mellitus and Masticatory Loading on Mandibular Growth in Growing Rats: A Longitudinal CBCT Study.

Biology·2026
Same journal

Data-Limited Stock Status Assessment of Bonga Shad, <i>Ethmalosa fimbriata</i> (Bowdich, 1825) and Lesser African Threadfin, <i>Galeoides decadactylus</i> (Bloch, 1795) in the Central Gulf of Guinea.

Biology·2026
Same journal

Gonadogenesis in the Bearded Dragon (<i>Pogona vitticeps</i>, Agamidae): A Comprehensive Histological Analysis from Gonadal Ridge Formation to Testicular and Ovarian Development.

Biology·2026
Same journal

The Programmable Microbiome: Integrative AI and Multi-Omics Frameworks for Precision T2DM Management.

Biology·2026
See all related articles

Related Experiment Video

Updated: Apr 29, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.4K

FLEXBAR-Flexible Barcode and Adapter Processing for Next-Generation Sequencing Platforms.

Matthias Dodt1, Johannes T Roehr2, Rina Ahmed3

  • 1Bioinformatics in Quantitative Biology, Berlin Institute for Medical Systems Biology at the Max Delbrück Centre for Molecular Medicine, Robert-Rössle-Straße 10, 13125 Berlin, Germany. matthias.dodt@googlemail.com.

Biology
|May 17, 2014
PubMed
Summary
This summary is machine-generated.

FLEXBAR software accurately identifies, sorts, and trims sequence tags from next-generation sequencing data. This flexible tool enhances biological data analysis, including read mapping and demultiplexing.

More Related Videos

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

921
Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer
07:59

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Published on: September 8, 2023

1.8K

Related Experiment Videos

Last Updated: Apr 29, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.4K
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
10:22

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

921
Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer
07:59

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Published on: September 8, 2023

1.8K

Area of Science:

  • Genomics
  • Bioinformatics

Background:

  • Next-generation sequencing generates vast amounts of short reads, often containing various sequence tags.
  • These tags include platform-specific adapters, experimental barcodes, and biological signals like splice leaders.

Purpose of the Study:

  • To introduce FLEXBAR, a software tool for flexible and accurate recognition, sorting, and trimming of sequence tags.
  • To provide a versatile solution for processing diverse sequencing data formats and experimental designs.

Main Methods:

  • FLEXBAR utilizes exact overlap sequence alignment for tag detection.
  • The software supports all current sequencing platforms, including color-space reads, and maintains read pairings.
  • It allows fine-grained adjustment of tag detection parameters and search regions, processing complex scenarios via a single command line.

Main Results:

  • Demonstrated utility of FLEXBAR in read mapping applications.
  • Showcased effectiveness in library demultiplexing.
  • Validated performance in splice leader detection.

Conclusions:

  • FLEXBAR offers a fast, precise, and flexible solution for essential sequence tag processing in quantitative and systems biology.
  • The software simplifies complex read processing, facilitating downstream genomic analyses.