Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Identification of D4 as a novel antiviral compound inhibiting hepatitis B virus surface antigen via TMEM40 upregulation.

Antiviral research·2026

Same author

An Optimized Strategy for Random Peptide Library Construction Facilitates Screening of HBc-Binding Peptides via Yeast Surface Display.

Biotechnology journal·2026

Same author

GFAT1 promotes the progression of hepatocellular carcinoma via enhancing the O-GlcNAcylation of VEZF1.

Cell death & disease·2025

Same author

SARS-CoV-2 N protein exerts antitumor effects in NSCLC by inducing DNA damage and augmenting chemotherapeutic sensitivity.

Medical oncology (Northwood, London, England)·2025

Same author

Angiotensin-converting enzyme 2 and hepatic SARS-CoV-2 infection: Regulation, association, and therapeutic implications.

World journal of gastroenterology·2025

Same author

Identification of virus epitopes and reactive T-cell receptors from memory T cells without peptide synthesis.

Communications biology·2024

Search research articles

Related Experiment Video

Updated: Apr 29, 2026

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

An improved method for simple and efficient hepatitis B virus genome cloning.

Zeng Tu¹, Yuan Hu², Lei Xu¹

¹Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China; Department of Microbiology, College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.

Journal of Virological Methods

|May 17, 2014

Summary

Researchers developed a new, efficient method for cloning full-length hepatitis B virus (HBV) genomes from patient samples. This technique simplifies genetic variation studies for HBV, aiding in understanding infection and treatment responses.

Keywords:

Diversity Full-length cloning Hepatitis B virus

More Related Videos

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

Related Experiment Videos

Last Updated: Apr 29, 2026

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

Area of Science:

Virology
Molecular Biology
Genetics

Background:

Hepatitis B virus (HBV) genetic variation influences infection progression and treatment outcomes.
Previous methods for studying HBV genetic diversity were limited to subgenomic regions or few full-length genomes.
Efficient cloning of full-length HBV genomes is crucial for comprehensive genetic analysis.

Purpose of the Study:

To present a simple, efficient, and restriction digestion-independent method for full-length HBV cloning from clinical samples.
To facilitate in-depth studies on HBV genetic variation and its clinical implications.

Main Methods:

Developed a novel cloning strategy using the amplified full-length HBV DNA from patient sera as megaprimers.
Constructed a new plasmid with homologous ends for HBV insertion, enabling DNA polymerase-mediated backbone extension.
Incorporated a LacZ gene fragment for blue-white screening to enhance cloning efficiency.

Main Results:

Achieved a high success rate of 90% for positive HBV clones from individual patient samples.
Demonstrated the method's efficiency and convenience for cloning full-length HBV from diverse clinical specimens.

Conclusions:

The presented method offers a simple, efficient, and convenient approach for full-length HBV cloning.
This technique is expected to significantly advance research into HBV genetic variation and its clinical relevance.