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Related Experiment Video

Updated: Apr 29, 2026

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

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Published on: January 5, 2024

Insertion sequence transposition determines imipenem resistance in Acinetobacter baumannii.

Han-Yueh Kuo¹, Kai-Chih Chang, Chih-Chin Liu

¹1 Department of Medicine, National Taiwan University Hospital Hsin-Chu Branch , Hsin-Chu City, Taiwan .

Microbial Drug Resistance (Larchmont, N.Y.)

|May 17, 2014

Summary

Imipenem exposure in Acinetobacter baumannii causes ISAba1 transposition, leading to blaOXA-95 gene overexpression and carbapenem resistance. This mechanism is crucial for understanding antibiotic resistance in this pathogen.

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Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Published on: March 10, 2014

Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Acinetobacter baumannii is a significant opportunistic pathogen known for its increasing antibiotic resistance.
Carbapenem antibiotics, including imipenem, are critical for treating A. baumannii infections.
Understanding the mechanisms of imipenem resistance is essential for developing effective therapeutic strategies.

Purpose of the Study:

To investigate the genomic mechanisms of imipenem resistance in Acinetobacter baumannii.
To identify genetic alterations responsible for phenotypic resistance following imipenem exposure.

Main Methods:

Multistep selection of imipenem-resistant mutants from a susceptible A. baumannii strain (ATCC 17978).
Antibiotic susceptibility testing and confirmation of mutants by pulsed-field gel electrophoresis.
Whole-genome sequencing of resistant mutants and PCR for genetic recombination confirmation.
Quantitative PCR to analyze blaOXA-95 gene expression.

Main Results:

Phenotypic resistance to carbapenems was observed in selected mutants.
Genomewide analysis revealed ISAba1 transposition upstream of the blaOXA-95 gene in all imipenem-selected mutants.
Imipenem exposure (≥0.5 mg/L) induced ISAba1 transposition.
A 200-fold increase in blaOXA-95 gene expression was detected, correlating with imipenem resistance.

Conclusions:

Imipenem exposure at 0.5 mg/L triggers ISAba1 transposition upstream of blaOXA-95.
Overexpression of blaOXA-95, mediated by ISAba1 transposition, is a key mechanism of imipenem resistance in A. baumannii.
This finding provides critical insights into carbapenem resistance development in A. baumannii.