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DNA-cholesterol barges as programmable membrane-exploring agents.

Alexander Johnson-Buck1, Shuoxing Jiang, Hao Yan

  • 1Single Molecule Analysis Group, Department of Chemistry, University of Michigan , 930 North University Avenue, Ann Arbor, Michigan 48109-1055, United States.

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Researchers developed cholesterol-labeled DNA origami barges that can embed in and move on lipid bilayers. These DNA nanodevices offer programmable control over membrane interactions and functions.

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Area of Science:

  • Biomolecular engineering
  • Nanotechnology
  • Membrane biophysics

Background:

  • Biological processes involve dynamic interactions at phospholipid membranes.
  • Synthetic mimics of membrane-associated systems are underdeveloped.
  • DNA nanotechnology offers precise control over nanoscale device construction.

Purpose of the Study:

  • To create and characterize synthetic DNA nanodevices for interaction with lipid bilayers.
  • To investigate the programmable behavior of DNA origami on supported lipid bilayers.
  • To explore the potential for membrane-bound molecular devices.

Main Methods:

  • Assembly and characterization of cholesterol-labeled DNA origami barges.
  • Single-particle fluorescence microscopy to track barge behavior.
  • Investigation of diffusion and association with supported lipid bilayers.

Main Results:

  • DNA origami barges stably embed in lipid bilayers.
  • Barge diffusion is dependent on cholesterol labeling and bilayer composition.
  • Super-resolution membrane mapping achieved through barge tracking.
  • Programmable control over cargo exchange, dissociation, and oligomerization demonstrated.

Conclusions:

  • Cholesterol-labeled DNA origami barges can reversibly associate with and diffuse on lipid bilayers.
  • These DNA nanodevices enable programmable control of membrane-associated functions.
  • The study opens new avenues for synthetic membrane-bound molecular devices.