A novel murine model for keratoprosthesis.

Area of Science:

• Ophthalmology
• Biomedical Engineering
• Surgical Innovation

Background:

• Keratoprosthesis (KPro) devices are crucial for vision restoration in severe corneal diseases.
• Existing KPro models may not fully replicate human clinical scenarios.
• A need exists for a reliable animal model to study KPro implantation and host responses.

Purpose of the Study:

• To establish a novel and reproducible murine model for keratoprosthesis implantation.
• To evaluate the feasibility and safety of a miniature keratoprosthesis (m-KPro) in a live animal model.
• To create a platform for future research on KPro-related complications and outcomes.

Main Methods:

• A custom-designed miniature keratoprosthesis (m-KPro) with a poly[methyl methacrylate] front and titanium back plate was utilized.
• BALB/c mice underwent syngeneic corneal transplantation with m-KPro implantation following extracapsular lens extraction.
• The cornea-device complex was secured with sutures, and animals were monitored for 8 weeks post-surgery.

Main Results:

• Successful implantation and retention of the m-KPro device were achieved in all 10 mice.
• No critical complications (e.g., endophthalmitis, corneal melting, extrusion) were observed.
• Mild to moderate corneal neovascularization in both donor and host corneas was noted throughout the follow-up period.

Conclusions:

• A robust murine model for keratoprosthesis implantation has been successfully established.
• This model provides a valuable experimental system for investigating host responses to KPro surgery.
• The model facilitates the study of potential complications and the efficacy of novel KPro designs.