Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sperm, egg, and embryo proteins critical for genetic adaptation of herring to low salinity in the Baltic Sea.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Targeting Interleukin-8-Mediated Cellular Crosstalk Reverses Hypertrophic Cardiomyopathy and Cardiac Fibrosis in Noonan Syndrome.

Circulation·2026
Same author

Mass spectrometric proteome profiling using a deep spectral library reveals homogenization of right and left atrial proteomes in persistent atrial fibrillation patients.

Cardiovascular research·2026
Same author

Dysferlin stabilizes membrane nanodomains of cardiomyocytes after myocardial infarction.

Scientific reports·2026
Same author

Distinct steps of nuclear maturation of human pre-60S complexes require the activity of GTPases including GNL3L.

Nucleic acids research·2026
Same author

The nuclear envelope protein TMEM209 is an integral component of the nuclear pore complex and interacts with Nup210.

Journal of cell science·2026
Same journal

Unveiling the post translational modification code in Alzheimer's pathogenesis: crosstalk between phosphorylation and acetylation.

Expert review of proteomics·2026
Same journal

Plasma proteome profiles of standard-risk and high-risk childhood acute lymphoblastic leukemia.

Expert review of proteomics·2026
Same journal

Potential proteomic biomarkers for monitoring clinical studies in Duchenne/Becker muscular dystrophy.

Expert review of proteomics·2026
Same journal

Proteomics-based approaches to neutrophil biology.

Expert review of proteomics·2026
Same journal

Deciphering mitochondrial metabolic vulnerabilities in ovarian clear cell carcinoma with mass spectrometry-based clinical proteomics.

Expert review of proteomics·2026
Same journal

The proteomic origin of the genetic code.

Expert review of proteomics·2026
See all related articles

Related Experiment Video

Updated: Apr 29, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.4K

Separation methodology to improve proteome coverage depth.

Christof Lenz1, Henning Urlaub

  • 1Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Expert Review of Proteomics
|May 17, 2014
PubMed
Summary
This summary is machine-generated.

In-depth proteomics, using advanced separation techniques, enhances the identification of low-abundance proteins. This approach improves proteome coverage depth for comprehensive system-wide cell biology studies.

Keywords:
data dependent acquisitionin-depth proteomicsorthogonal and multidimensional protein and peptide separationsingle shot proteomics

More Related Videos

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.6K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.4K

Related Experiment Videos

Last Updated: Apr 29, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.4K
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

2.6K
Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
09:28

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

3.4K

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • System-wide Cell Biology

Background:

  • In-depth proteomics aims to increase proteome coverage depth.
  • Current methods are crucial for identifying low-copy-number proteins.
  • This field is vital for correlating gene expression with cellular proteomes.

Purpose of the Study:

  • To review recent advances in in-depth proteomics.
  • To highlight improvements in peptide and protein separation technologies.
  • To discuss methodologies for achieving maximum protein coverage.

Main Methods:

  • Bottom-up proteomics approach.
  • Application of diverse separation techniques.
  • Single injection analyses using long reversed-phase liquid chromatography (LC) separations.
  • Combination of orthogonal protein and peptide separation methods.

Main Results:

  • Recent advances focus on peptide and protein separation technologies.
  • Single-shot proteomics utilizes long LC separations for peptide analysis.
  • Orthogonal separation strategies enhance overall protein identification.
  • Improved methodologies are enabling deeper proteome coverage.

Conclusions:

  • In-depth proteomics has become a significant area of research.
  • Advanced separation techniques are key to enhancing proteome coverage.
  • These improved methods are increasingly adopted in laboratories worldwide.