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Updated: Apr 29, 2026

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
Published on: October 6, 2022
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Randomly arrayed G-quadruplexes for label-free and real-time assay of enzyme activity
Zhuoliang Liu1, Wang Li, Zhou Nie
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. niezhou.hnu@gmail.com.
Abstract:
Randomly arrayed G-quadruplexes can serve as an efficient peroxidase-mimic DNAzyme and provide a novel and facile method to detect terminal deoxynucleotidyl transferase (TdT). Moreover, this G-rich sequence binding to the thioflavin T (ThT) dye can be applied in real-time fluorescent detection of TdT activity.

