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Updated: Apr 29, 2026

Microdialysis of Ethanol During Operant Ethanol Self-administration and Ethanol Determination by Gas Chromatography
Published on: September 5, 2012
Ethanol determination using pressure mobilization and free solution capillary electrophoresis by photo-oxidation
James D Oliver1, Marianne Gaborieau2, Patrice Castignolles1
1University of Western Sydney (UWS), Australian Centre for Research on Separation Science (ACROSS), School of Science and Health, Locked Bag 1797, Penrith, NSW 2751, Australia.
Abstract:
Free solution capillary electrophoresis (CE) can separate and quantify carbohydrates using a simple direct UV detection based on a photo-oxidation reaction taking place in the detection window without any labeling. Ethanol interferes with this photo-oxidation reaction. We thus present the first detection and quantification of ethanol using either a simple pressure mobilization set-up or CE. Ethanol can be detected down to 34.9mgL(-1) and quantified in the range 117-1850mgL(-1) through the interference with photo-oxidization of 2gL(-1) sucrose. CE can thus separate and quantify both carbohydrates and ethanol, for example to monitor a lignocellulosic fermentation process. The method is not limited to ethanol and applies to alkyl amines and other alcohols and likely to most molecules possessing the ability to react with free radicals assuming they can be sufficiently separated from each other.
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