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Synthesis and potent ionophoric activity of a squaramide-linked bis(choloyl) conjugate
Li-Qun Deng1, Yong-Ming Lu1, Chun-Qiong Zhou1
1School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China.
Abstract:
A squaramide-linked bis(choloyl) conjugate was synthesized and fully characterized on the basis of NMR ((1)H and (13)C) and ESI MS (LR and HR) data. Fluorescence and chloride ion selective electrode assays indicate that this compound exhibits potent ionophoric activity across egg-yolk L-α-phosphatidylcholine-based liposomal membranes, presumably via an anion-modulating anion-cation co-transport/symport process. A Hill analysis reveals that three molecules of this compound are assembled into the transport-active species.
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