Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.5K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.5K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.2K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.2K
Master Transcription Regulators02:23

Master Transcription Regulators

6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

2.3K
2.3K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A telomere-to-telomere reference genome assembly of the Hypomesus nipponensis.

Scientific data·2026
Same author

Basal level of ATF4 promotes T cell readiness for activation-induced proliferation.

iScience·2026
Same author

Telomere-to-telomere genome assembly of Phoxinus lagowskii.

Scientific data·2025
Same author

Impact of Seasonal Atmospheric Factors and Photoperiod on Floral Biology, Plant-Pollinator Interactions, and Plant Reproduction on <i>Turnera ulmifolia</i> L. (Passifloraceae).

Biology·2025
Same author

Targeting erbB Pathways in Breast Cancer: Dual Kinase Inhibition for Brain Metastasis and Prevention of p185HER2/Neu Tumor Development.

Breast cancer (Dove Medical Press)·2024
Same author

Anti-inflammatory Potential of <i>Costus speciosus</i> rhizome Bioactive Phytochemicals: A Combined GC-MS and Computational Approach Targeting TLR-4 Signaling.

Current computer-aided drug design·2024

Related Experiment Video

Updated: Apr 29, 2026

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
08:13

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

6.2K

Dynamic interactions between TIP60 and p300 regulate FOXP3 function through a structural switch defined by a single

Yan Xiao1, Yasuhiro Nagai1, Guoping Deng1

  • 1Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Cell Reports
|May 20, 2014
PubMed
Summary
This summary is machine-generated.

The histone acetyltransferases TIP60 and p300 cooperatively regulate the transcriptional factor FOXP3, which is crucial for T regulatory cell function. Their interaction controls FOXP3 activity, impacting immune response regulation and preventing autoimmune disease.

More Related Videos

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

6.8K
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
11:46

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Published on: May 26, 2014

22.7K

Related Experiment Videos

Last Updated: Apr 29, 2026

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60
08:13

Live-imaging of Breast Epithelial Cell Migration After the Transient Depletion of TIP60

Published on: December 7, 2017

6.2K
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

6.8K
Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer
11:46

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

Published on: May 26, 2014

22.7K

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • FOXP3 is a key transcriptional factor for T regulatory cells, essential for immune response regulation.
  • FOXP3 activity is modulated by posttranslational modifications, including acetylation by histone acetyltransferases (HATs).
  • TIP60 and p300 are identified as critical HATs involved in regulating FOXP3 function.

Purpose of the Study:

  • To elucidate the cooperative mechanism between TIP60 and p300 in regulating FOXP3 activity.
  • To investigate the functional consequences of TIP60 and p300 interaction on FOXP3 acetylation and T regulatory cell homeostasis.
  • To understand the role of TIP60 in maintaining the peripheral Treg population and preventing autoimmune diseases.

Main Methods:

  • Co-immunoprecipitation assays to study protein-protein interactions between TIP60, p300, and FOXP3.
  • In vitro acetylation assays to assess the HAT activity of TIP60 and p300 on FOXP3.
  • Site-directed mutagenesis to identify key acetylation sites on FOXP3 and TIP60.
  • Conditional knockout models to evaluate the in vivo function of TIP60 in T regulatory cells.

Main Results:

  • TIP60 and p300 interact to activate TIP60, leading to the acetylation of TIP60 at K327, which acts as a molecular switch.
  • This switch allows TIP60 to bind and acetylate FOXP3, enhancing its transcriptional activity.
  • Cooperative action of TIP60 and p300 is necessary for maximal induction of FOXP3 activity.
  • Conditional knockout of TIP60 in Treg cells results in a diminished Treg population and a scurfy-like autoimmune phenotype.

Conclusions:

  • TIP60 and p300 function cooperatively to regulate FOXP3 acetylation and activity, playing a vital role in immune homeostasis.
  • TIP60-mediated acetylation of FOXP3 is critical for maintaining the stability and function of T regulatory cells.
  • Disruption of TIP60 function leads to Treg depletion and the development of fatal autoimmune disease, highlighting its therapeutic relevance.