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Updated: Apr 29, 2026

Neonatal Pial Surface Electroporation

Neonatal Pial Surface Electroporation

Published on: May 7, 2014

Neonatal pial surface electroporation.

Rachelle Levy¹, Jessica Molina², Moise Danielpour¹

¹Department of Neurosurgery, Regenerative Medicine Institute, Cedars-Sinai Medical Center.

Journal of Visualized Experiments : Jove

|May 20, 2014

Summary

This summary is machine-generated.

Researchers developed a new electroporation method to efficiently study pial surface progenitor cells. This technique allows for rapid genetic manipulation and lineage tracing, advancing neurogenesis research.

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Published on: September 27, 2024

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Last Updated: Apr 29, 2026

Neonatal Pial Surface Electroporation

Neonatal Pial Surface Electroporation

Published on: May 7, 2014

Neonatal Subventricular Zone Electroporation

Neonatal Subventricular Zone Electroporation

Published on: February 11, 2013

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization

Author Spotlight: Optimizing Porous Substrate Electroporation Through Micro and Nanochannels for Enhanced Monitoring and Intermediate Stage Characterization

Published on: September 27, 2024

Area of Science:

Neuroscience
Developmental Biology
Stem Cell Research

Background:

The pial surface is a crucial germinal niche for neurogenesis and gliogenesis throughout development and after injury.
Previous methods for genetic interrogation of these progenitor cells were limited by specificity and time-consuming viral production.

Purpose of the Study:

To develop an efficient, rapid, and simple technique for the genetic manipulation of pial surface progenitor cells.
To overcome limitations in studying these cells and facilitate progress in neurogenesis research.

Main Methods:

Adaptation of an existing electroporation technique.
Application of electroporation to the pial surface for genetic manipulation.

Main Results:

The adapted electroporation approach provides efficient genetic labeling and manipulation of pial surface progenitors.
This method is time-saving and economical compared to previous techniques.

Conclusions:

Pial surface electroporation is a valuable tool for studying progenitor cells in this niche.
This technique will accelerate research into neuro- and gliogenesis at the pial surface.