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Skin blotting: a noninvasive technique for evaluating physiological skin status
Takeo Minematsu1, Motoko Horii, Makoto Oe
1Takeo Minematsu, PhD, is a Project Lecturer, Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Japan. Motoko Horii, MHS, is an Assistant Professor, Faculty of Nursing, Josai International University, Chiba, Japan. Makoto Oe, PhD, is a Project Lecturer, Department of Advanced Nursing Technology, Graduate School of Medicine, The University of Tokyo. Junko Sugama, PhD, is a Professor, Department of Clinical Nursing, Graduate School of Medical Science, Kanazawa University, Ishikawa, Japan. Yuko Mugita, MHS, is a Doctoral Student; Lijuan Huang, PhD, is a Project Researcher; Gojiro Nakagami, PhD, is a Lecturer; and Hiromi Sanada, PhD, is a Professor, all at the Department of Gerontological Nursing/Wound Care Management, Graduate School of Medicine, The University of Tokyo, Japan.
A novel skin blotting technique noninvasively assesses skin physiology by detecting protein leakage. This method shows promise for diagnosing skin disorders and monitoring inflammatory responses, with potential applications in both research and clinical settings.
Area of Science:
- Biomedical Engineering
- Dermatology
- Biochemistry
Background:
- Skin assessment is crucial for identifying dermatological issues.
- Current noninvasive methods lack the ability to monitor skin's physiological status.
- A novel technique is needed to assess skin physiology noninvasively.
Purpose of the Study:
- To develop and validate a novel noninvasive skin assessment technique called skin blotting.
- To investigate the leakage of secreted proteins from the skin following overhydration.
- To evaluate the clinical applicability of skin blotting for skin assessment.
Main Methods:
- Skin blotting involves collecting proteins on a nitrocellulose membrane and subsequent immunostaining.
- Fluorescein-conjugated dextran (F-DEX) distribution was analyzed in mice skin.
- Correlations between inflammatory cytokine secretion, UV irradiation, and body mass index were examined.
Main Results:
- F-DEX distribution revealed leakage through transfollicular and transepidermal routes in mice.
- Skin blotting detected tumor necrosis factor secretion in response to UV irradiation in mice.
- Follicular tumor necrosis factor correlated with body mass index in obese humans, supporting clinical applicability.
Conclusions:
- Skin blotting is a novel noninvasive technique for assessing skin physiology.
- This method has potential as a predictive and diagnostic tool for various skin disorders.
- Skin blotting offers a new avenue for understanding skin health and disease.
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