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Optimization strategies for the polymerase chain reaction.

¹Perkin-Elmer Corporation, Norwalk, CT 06859-0251.

|July 1, 1989

Summary

Polymerase chain reaction (PCR) is a flexible molecular biology technique for amplifying DNA sequences. This method is crucial for diverse applications, from genetic disease research to infectious microorganism detection.

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Area of Science:

Molecular Biology
Biochemistry

Background:

Polymerase chain reaction (PCR) is a fundamental technique in molecular biology research.
The PCR method enables in vitro enzymatic amplification of specific genomic or cloned DNA target sequences using oligonucleotide primers.

Purpose of the Study:

To present current recommendations for optimizing the GeneAmp polymerase chain reaction (PCR) process.
To highlight the flexibility and diverse applications of PCR in scientific research.

Main Methods:

The study focuses on the biochemical and biophysical parameters influencing PCR optimization.
Recommendations are based on collaborative research between Cetus and Perkin-Elmer.

Main Results:

PCR is a robust and flexible technique with numerous variations like inverse PCR, anchored PCR, and RNA-PCR.
Current applications span direct sequencing, genomic cloning, DNA typing, and disease research.

Conclusions:

The GeneAmp PCR process is a vital tool in molecular biology laboratories.
Ongoing research continues to refine our understanding and optimization of PCR techniques.

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