Related Experiment Video
Updated: Aug 3, 2026
09:00
Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology
Published on: March 27, 2018
Localization of terminal complement components S-protein and SP-40,40 in renal biopsies
B F Murphy1, D J Davies, W Morrow
1Department of Anatomical Pathology, St Vincent's Hospital, Melbourne.
Pathology
|October 1, 1989
Summary
The terminal complement complex, specifically the SC5b-9 form, is present in various kidney injury sites. This complex, including SP-40,40, correlates with renal damage severity.
Area of Science:
- Nephrology
- Immunology
- Pathology
Background:
- The terminal complement complex is linked to glomerular injury in glomerulonephritis.
- The inactive SC5b-9 form, containing S-protein and SP-40,40, may be prevalent in human disease.
Purpose of the Study:
- To investigate the incidence and co-localization of SC5b-9 components (C6, C9, S-protein, SP-40,40) in renal biopsies.
- To determine the relationship between SC5b-9 deposition and renal injury markers.
Main Methods:
- Immunofluorescence examination of renal biopsies.
- Detection of SC5b-9 components (C6, C9, S-protein, SP-40,40), C3, and immunoglobulins.
Main Results:
- SC5b-9 components were found in arteries, arterioles, tubular basement membranes, and glomerulosclerosis areas in all biopsies.
- Deposition correlated with renal injury and sometimes co-localized with C3, but not immunoglobulin.
- SP-40,40 distribution mirrored S-protein, C6, and C9, confirming its presence in the SC5b-9 complex.
Conclusions:
- The terminal complement complex in the kidney exists partly as SC5b-9 in both immune deposits and areas of renal injury.
- SP-40,40 is consistently found within the SC5b-9 complex across all renal disease types studied.
