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A protective chimeric antibody to tick-borne encephalitis virus.
Ivan K Baykov1, Andrey L Matveev1, Oleg V Stronin2
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, prosp. Lavrentieva 8, Novosibirsk 630090, Russia.
Vaccine
|May 20, 2014
Summary
The chimeric antibody ch14D5a demonstrated high efficacy in preventing tick-borne encephalitis virus (TBEV) infection post-exposure. This TBEV glycoprotein E-specific antibody offers promising therapeutic potential for TBEV prevention.
Area of Science:
- Virology
- Immunology
- Biotechnology
Background:
- Tick-borne encephalitis virus (TBEV) poses a significant public health threat.
- Effective post-exposure prophylaxis strategies against TBEV are crucial.
Purpose of the Study:
- To evaluate the efficacy of mouse monoclonal antibodies (mAbs) against TBEV glycoprotein E for post-exposure prophylaxis.
- To develop and characterize a potent chimeric antibody for TBEV treatment.
Main Methods:
- Screening of multiple mAbs, including mAb14D5, for TBEV neutralization.
- Construction and characterization of chimeric antibodies (ch14D5a, ch14D5b) using hybridoma-derived immunoglobulin chains.
- Affinity determination using Surface Plasmon Resonance (SPR) via ProteOn biosensor.
- In vitro neutralization assay (focus reduction neutralization test) to determine IC50.
- In vivo efficacy study in mice challenged with TBEV.
Main Results:
- mAb14D5 exhibited the highest activity among tested mAbs.
- Chimeric antibody ch14D5a showed a high affinity (2.6 × 10^10 M⁻¹) and potent in vitro neutralization (IC50 = 0.04 μg/ml).
- In vivo, ch14D5a provided 100% survival in mice infected with a lethal dose of TBEV.
Conclusions:
- Chimeric antibody ch14D5a is a highly effective candidate for TBEV post-exposure prophylaxis.
- ch14D5a demonstrates significant potential for the development of novel TBEV prevention and therapeutic agents.
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