Related Experiment Video
Updated: Apr 29, 2026

Fluorescent Paper Strips for the Detection of Diesel Adulteration with Smartphone Read-out
Published on: November 9, 2018
Low level detection of Benzene in Food Grade Hexane by Ultraviolet Spectrophotometry
R Emmandi1, M I S Sastry1, M B Patel1
1Research & Development Center, Indian Oil Corporation Limited, Sector-13, Faridabad 121007, Haryana, India.
Abstract:
A simple, sensitive, and accurate Ultraviolet Spectrophotometric method has been developed and validated for the determination of Benzene in Food Grade Hexane. Benzene in spectroscopic grade Hexane shows vibrational fine structure having four well resolved peaks. In the wavelength range 240-270nm, peak at 255nm is considered for the method development. Beer's law was obeyed in the concentration range of 0.6-10.0μLL(-1), with correlation coefficient, 0.9999, detection limit 0.2μLL(-1) and quantitation limit 0.6μLL(-1) are established. Percentage recovery studies showed that the method was not affected by the presence of other solvents having the similar boiling range with Hexane. The method was validated by determining its accuracy and precision which proves suitability of the developed method for the routine determination of Benzene in Food Grade Hexane. The proposed method has been applied successfully for the analysis of the Food Grade Hexane.
More Related Videos
05:34Efficient Synthesis of Polyfunctionalized Benzenes in Water via Persulfate-promoted Benzannulation of α,β-Unsaturated Compounds and Alkynes
Published on: December 16, 2019
04:39A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
Published on: June 13, 2025
Related Concept Videos
UV–Vis Spectrum
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar...
UV–Vis Spectrometers
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
NMR Spectroscopy of Benzene Derivatives
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is...
UV–Vis Spectroscopy: Woodward–Fieser Rules