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Updated: Apr 29, 2026
In Vivo Imaging Systems IVIS Detection of a Neuro-Invasive Encephalitic Virus
Published on: December 2, 2012
In vivo imaging in an ABSL-3 regional biocontainment laboratory
Charles A Scanga1, Brian J Lopresti, Jaime Tomko
1Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Abstract:
The Regional Biocontainment Laboratory (RBL) at the University of Pittsburgh is a state-of-the-art ABSL-3 facility that supports research on highly pathogenic viruses and bacteria. Recent advances in radiologic imaging provide several noninvasive, in vivo imaging modalities that can be used to longitudinally monitor animals following experimental infection or vaccination. The University of Pittsburgh RBL provides digital radiography, bioluminescence imaging, and PET/CT. Operating these platforms in an ABSL-3 poses unique challenges. This review will discuss the development and refinement of these imaging platforms in high containment, emphasizing specific challenges and how they were overcome.
