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Updated: Apr 29, 2026

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance
Published on: October 21, 2021
Analysis of calcium signals in steering neuronal growth cones in vitro
Hiroki Akiyama1, Hiroyuki Kamiguchi
1Laboratory for Neuronal Growth Mechanisms, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Abstract:
Calcium imaging allows us to measure the spatial and temporal changes in intracellular calcium concentration in living cells. Localized calcium elevation often functions as the polarizing signal during guided migration including axon guidance. In this chapter, we describe a protocol to quantitatively monitor the spatiotemporal dynamics of calcium signals in neuronal growth cones in the presence of an extracellular concentration gradient of axon guidance cue.

