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Related Experiment Video

Updated: Apr 29, 2026

Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

Published on: October 30, 2018

Growth cone collapse assay.

Geoffrey M W Cook¹, Prem Jareonsettasin, Roger J Keynes

¹Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge, CB2 3DY, UK.

Methods in Molecular Biology (Clifton, N.J.)

|May 20, 2014

Summary

This summary is machine-generated.

The growth cone collapse assay identifies axonal repellents by observing changes in neuronal growth cone morphology. This method is crucial for purifying molecules that inhibit axon growth.

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Last Updated: Apr 29, 2026

Visualizing Axonal Growth Cone Collapse and Early Amyloid β Effects in Cultured Mouse Neurons

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Published on: October 30, 2018

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Published on: February 20, 2008

Area of Science:

Neuroscience
Cell Biology

Background:

Axonal guidance is critical for neural development.
Identifying molecules that repel axonal growth is essential for understanding neural circuit formation.

Purpose of the Study:

To describe the growth cone collapse assay.
To highlight its utility in detecting and purifying axonal repellents.

Main Methods:

Incorporating tissue extracts or purified molecules into liposomes or aqueous solutions.
Applying these to cultured neuronal growth cones.
Assessing morphological changes via microscopy after incubation.

Main Results:

The assay quantifies growth cone collapse, indicated by loss of filopodia and lamellipodia.
This provides a measurable response to repellent molecules.

Conclusions:

The growth cone collapse assay is an invaluable tool for discovering and isolating axonal repellent factors.
It enables the study of molecules influencing axon growth and guidance.