Related Experiment Video
Updated: Apr 29, 2026
Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration
Published on: November 2, 2020
Application of tissue clearing and light sheet fluorescence microscopy to assess optic nerve regeneration in
Xueting Luo1, Benjamin Yungher, Kevin K Park
1Department of Neurosurgery, Miami Project to Cure Paralysis, Miller School of Medicine, University of Miami, Park Lab, LPLC, RM 3-01, 1095 NW 14th Terrace, Miami, FL, 33136, USA.
Abstract:
Optic nerve crush injury, as a model to study central nervous system (CNS) injury, is widely used to assess potential therapeutic strategies, aimed at promoting axon regeneration and neuronal survival. Traditional methods to evaluate optic nerve regeneration rely on histological sectioning. However, tissue sectioning results in inevitable loss of three-dimensional (3D) information, such as axonal trajectories and terminations. Here we describe a protocol for whole-tissue assessment of optic nerve regeneration in adult mice without the need for histological sectioning.
