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Updated: Apr 29, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
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Expression patterns of microRNA-218 and its potential functions by targeting CIP2A and BMI1 genes in melanoma

Yanping Wei1, Yuwen Du, Xiaonan Chen

  • 1Department of Dermatology, The People's Hospital of Jiaozuo City, Jiaozuo, 454000, Henan, China.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|May 20, 2014
PubMed

Insights

MicroRNAs (miRNAs), specifically miR-218, are downregulated in melanoma. This microRNA targets oncogenes CIP2A and BMI1, regulating melanoma cell proliferation, migration, and invasion.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Melanoma is an aggressive skin cancer known for its resistance to chemotherapy.
  • MicroRNAs (miRNAs) are implicated in the development and progression of malignant melanoma.
  • Understanding miRNA roles is crucial for developing novel melanoma therapies.

Purpose of the Study:

  • To investigate the expression level and function of miR-218 in melanoma.
  • To identify the direct targets of miR-218 in melanoma cells.
  • To elucidate the role of miR-218 in regulating melanoma cell biological processes.

Main Methods:

  • Analysis of miR-218 expression in melanoma patients and cell lines.
  • Bioinformatic analysis using TargetScan and miRanda.
  • Dual-Luciferase reporter assays, qRT-PCR, and Western blot to validate miR-218 targets.
  • Functional assays assessing proliferation, cell cycle, migration, and invasion.

Main Results:

  • miR-218 expression was found to be downregulated in melanoma.
  • miR-218 was confirmed to directly target the 3'-UTR of oncogenes CIP2A and BMI1.
  • Overexpression of miR-218 inhibited melanoma cell proliferation, migration, and invasion.
  • Knockdown of CIP2A and BMI1 mimicked the effects of miR-218 overexpression.

Conclusions:

  • miR-218 acts as a tumor suppressor in melanoma by targeting CIP2A and BMI1.
  • Restoring miR-218 levels could be a potential therapeutic strategy for melanoma.
  • This study highlights the critical role of miR-218 in melanoma pathogenesis.

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