Related Experiment Video
Updated: Apr 29, 2026
Quantifying Antibody-Dependent Cellular Cytotoxicity in a Tumor Spheroid Model: Application for Drug Discovery
Published on: April 26, 2024
Quantifying the autophagy-triggering effects of drugs in cell spheroids with live fluorescence microscopy
Nariman Ansari1, Stefanie Hardung, Katharina Hötte
1Physical Biology Group, Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt am Main, Max-von-Laue-Str, 15D-60438, Frankfurt am Main, Germany.
Abstract:
We present a 3D assay for the quantification of the autophagic flux in live cell spheroids by using the fluorescent reporter mRFP-GFP-LC3. The protocol describes the formation of the spheroids from the astrocytoma cell line U343, live long-term 3D fluorescence imaging of drug-treated spheroids, and the image processing workflow required to extract quantitative data on the autophagic flux.
