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Updated: Apr 29, 2026
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Genome Editing with CompoZr Custom Zinc Finger Nucleases ZFNs
Published on: June 14, 2012
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Gene disruption using zinc finger nuclease technology
Sara Granja1, Ibtissam Marchiq, Fátima Baltazar
1Surgical Sciences Domain, Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus de Gualtar, Braga, 4710-057, Portugal.
Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2014
Summary
Zinc finger nucleases efficiently disrupt genes by creating targeted DNA breaks. This study demonstrates successful gene disruption of CD147/basigin in human cancer cells for therapeutic research.
Area of Science:
- Molecular Biology
- Genetics
- Cancer Research
Background:
- Zinc finger nucleases (ZFNs) are engineered DNA-binding proteins that facilitate targeted genome modification.
- ZFNs induce double-strand breaks, which are repaired by cellular mechanisms, leading to gene alterations.
- This technology offers a powerful tool for gene disruption in various biological systems.
Purpose of the Study:
- To describe the application of ZFNs for gene disruption.
- To investigate the disruption of the CD147/basigin gene using ZFN technology.
- To demonstrate the efficacy of ZFNs in a human cancer cell line.
Main Methods:
- Utilized zinc finger nucleases (ZFNs) as a gene-editing tool.
- Induced targeted DNA double-strand breaks at specific genomic loci.
- Employed nonhomologous end joining (NHEJ) repair pathway for gene alteration.
- Applied the technique to a human cancer cell line.
Main Results:
- Successfully disrupted the CD147/basigin gene in a human cancer cell line.
- Demonstrated the efficiency of ZFNs in achieving targeted gene disruption.
- Confirmed alterations in the genome following ZFN treatment.
Conclusions:
- ZFN technology provides a highly efficient method for gene disruption.
- CD147/basigin disruption in cancer cells is feasible using ZFNs.
- This approach holds potential for novel therapeutic applications in cancer treatment.
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