Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

3.5K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.5K
Bone Formation by Intramembranous Ossification01:29

Bone Formation by Intramembranous Ossification

16.1K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
16.1K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

3.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.9K
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

14.6K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
14.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biofunctionalized Vascular Access Graft Improves Patency and Endothelialization in a Porcine Arteriovenous Model.

Journal of functional biomaterials·2026
Same author

Bulk RNA Sequencing Reveals Signature Differences in Key Cell Signaling Pathways Between Porcine Venous and Arterial Smooth Muscle Cells.

International journal of molecular sciences·2025
Same author

Uremic serum exposure leads to differential phenotypic switch in porcine arterial and venous smooth muscle cells.

American journal of physiology. Renal physiology·2025
Same author

Effects of weight-loss interventions on bone health in people living with obesity.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2025
Same author

Omics characteristics and epigenetic modifications of adipose-derived stem cells.

The Journal of biological chemistry·2025
Same author

Building a collaborative ecosystem across the IDeA-CTR networks in response to a public health emergency.

Journal of clinical and translational science·2025

Related Experiment Video

Updated: Apr 29, 2026

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
10:32

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Published on: May 19, 2023

4.0K

IGFBP-2 directly stimulates osteoblast differentiation.

Gang Xi1, Christine Wai, Victoria DeMambro

  • 1Department of Medicine, University of North Carolina, Chapel Hill, NC, USA.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|May 20, 2014
PubMed
Summary

Insulin-like growth factor binding protein 2 (IGFBP-2) stimulates osteoblast differentiation and bone mass acquisition. This process involves IGFBP-2 binding to RPTPβ via its heparin-binding domain-1, activating AKT signaling.

Keywords:
IGFBP-2OSTEOBLAST DIFFERENTIATIONPTENRPTPβpAKT

More Related Videos

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

7.7K
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

13.2K

Related Experiment Videos

Last Updated: Apr 29, 2026

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
10:32

Author Spotlight: Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate

Published on: May 19, 2023

4.0K
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

7.7K
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
10:23

Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach

Published on: August 26, 2013

13.2K

Area of Science:

  • Bone Biology
  • Cellular Differentiation
  • Molecular Mechanisms

Background:

  • Insulin-like growth factor binding protein 2 (IGFBP-2) is crucial for bone mass development in mice.
  • The precise mechanisms underlying IGFBP-2's role in bone formation remain undefined.
  • Understanding IGFBP-2's function is key to addressing bone mass acquisition disorders.

Purpose of the Study:

  • To investigate the role of IGFBP-2 in stimulating osteoblast differentiation.
  • To elucidate the molecular mechanisms by which IGFBP-2 influences osteoblast activity.
  • To identify the specific domains of IGFBP-2 involved in regulating bone formation.

Main Methods:

  • Utilized MC-3T3 preosteoblasts and primary calvarial osteoblasts from IGFBP-2 knockout mice.
  • Performed gene knockdown and overexpression studies for IGFBP-2 and RPTPβ.
  • Examined the interaction between IGFBP-2 and receptor tyrosine phosphatase β (RPTPβ).
  • Investigated AKT activation and employed IGFBP-2 mutants, including a heparin-binding domain-1 (HBD-1) mutant.

Main Results:

  • IGFBP-2 knockdown delayed osteoblast differentiation and reduced osteocalcin expression.
  • IGFBP-2 overexpression accelerated differentiation and increased osteoblast numbers.
  • IGFBP-2's ability to stimulate differentiation and AKT activation depends on its interaction with RPTPβ.
  • The heparin-binding domain-1 of IGFBP-2 is essential for mediating these effects.

Conclusions:

  • IGFBP-2 significantly stimulates osteoblast differentiation.
  • The interaction between IGFBP-2's HBD-1 and RPTPβ is critical for this stimulatory effect.
  • IGFBP-2-mediated osteoblast differentiation is a key mechanism for regulating bone mass acquisition in mice.