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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Apr 29, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

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Published on: February 1, 2014

Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic E. coli Detection

Joanna R Klein¹

¹University of Northwestern - St. Paul, St. Paul, MN 55113.

Journal of Microbiology & Biology Education

|May 20, 2014

Summary

No abstract available in PubMed .

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