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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

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Towards Engineered Processes for Sequencing-Based Analysis of Single Circulating Tumor Cells.

Viktor A Adalsteinsson1, J Christopher Love1

  • 1The David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA ; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA ; The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02412, USA.

Current Opinion in Chemical Engineering
|May 20, 2014
PubMed
Summary

Analyzing single circulating tumor cells (CTCs) via sequencing can transform cancer care. Developing integrated processes is crucial to overcome challenges in CTC yield, identification, and DNA analysis for routine clinical use.

Keywords:
Microfluidicscancer genomicssingle-cell sequencing

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Area of Science:

  • Oncology
  • Genomics
  • Biotechnology

Background:

  • Single circulating tumor cells (CTCs) offer insights into metastatic cancer.
  • Existing technologies for CTC analysis require integration for routine clinical application.

Purpose of the Study:

  • To highlight the need for integrated processes for systematic single CTC analysis.
  • To address challenges in CTC enrichment, identification, and DNA sequencing.

Main Methods:

  • Review of existing technologies for CTC enrichment, identification, recovery, and sequencing.
  • Discussion of challenges including CTC yield, viability, DNA integrity, and amplification bias.
  • Exploration of advances in single-cell analysis and nanoscale technologies.

Main Results:

  • Current methods for single CTC analysis face challenges in efficiency and robustness.
  • Integrated platforms are emerging to streamline multiple unit operations.
  • Advances in technology offer solutions for overcoming current limitations.

Conclusions:

  • Optimized, integrated processes are essential for routine single CTC sequencing.
  • Selection and adaptation of technologies are key for successful implementation.
  • This approach holds potential to revolutionize understanding and treatment of metastatic cancers.