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Transient increase in circulating myeloid-derived suppressor cells after partial bladder outlet obstruction
Wei-Yu Lin1, Ching Chuan Hsieh1, Teng-Yao Yang1
1Division of Urology, Department of Surgery (WYL, LYH, YPL), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Surgery (CCH), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Department of Cardiology (TYY), Chang Gung Memorial Hospital, Chia-Yi, Taiwan, Republic of China; Chang Gung University of Science and Technology (WYL), Chia-Yi, Taiwan, Republic of China; Department of Medicine and Graduate Institute of Clinical Medical Sciences, College of Medicine, Chang Gung University (WYL), Taoyuan, Taiwan, Republic of China; Department of Nutrition and Health Sciences, College of Health Science, Innovate Research Center of Medicine, Chang Jung Christian University (MLC), Tainan, Taiwan, Republic of China; Department of Medicine, Mackay Medical College (YHW), New Taipei City, Taiwan, Republic of China; Albany College of Pharmacy and Health Sciences and Stratton Veterans Affairs Medical Center, Albany (RML), New York.
Purpose:
Partial bladder outlet obstruction causes a significant increase in tissue and systemic oxidative stress markers and tissue inflammatory cytokine levels. Myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone are believed to be associated with oxidative stress and inflammation. We investigated alterations in plasma myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone levels in partial bladder outlet obstruction and after its reversal.
Materials And Methods:
Rats with surgically induced partial bladder outlet obstruction were divided into 4 groups of 3 each, including sham treated, 4-week obstruction, and 4 and 8-week obstruction with relief. Plasma levels of circulating myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone were assessed by flow cytometry or enzyme-linked immunosorbent assay.
Results:
The circulating myeloid-derived suppressor cell level was markedly increased in the obstruction group compared to the sham treated group and it returned to normal in the 4 and 8-week obstruction with relief groups. Plasma IFN-γ, IL-10 and aldosterone were similarly increased in the obstruction group and returned to normal in the 4 and 8-week obstruction with relief groups.
Conclusions:
Levels of circulating myeloid-derived suppressor cells, IFN-γ, IL-10 and aldosterone were increased in rats with partial bladder outlet obstruction but returned to normal after reversal. This suggests that an increase in these parameters may be a good predictive indicator of patients at increased risk for urinary symptoms.
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