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PDGF-BB inhibits intervertebral disc cell apoptosis in vitro
Steven M Presciutti1, David N Paglia, Teja Karukonda
1Departments of Orthopaedic Surgery, University of Connecticut Health Center, 263 Farmington Avenue, MARB Building, 4th Floor, Farmington, Connecticut, 06030.
Summary
Recombinant human platelet-derived growth factor-BB (rhPDGF-BB) inhibits apoptosis and promotes proliferation in degenerating intervertebral disc (IVD) cells. This suggests rhPDGF-BB may offer a future biologic treatment for early degenerative disc disease.
Area of Science:
- Biochemistry
- Cell Biology
- Regenerative Medicine
Background:
- Intervertebral disc (IVD) degeneration causes spinal issues and back pain.
- Platelet-derived growth factor-BB (PDGF-BB) has known cell growth and survival effects.
Purpose of the Study:
- To investigate if recombinant human PDGF-BB (rhPDGF-BB) can delay IVD cell degeneration by inhibiting apoptosis.
- To explore rhPDGF-BB's effects on IVD cell proliferation, differentiation, and gene expression.
Main Methods:
- Human IVD cells from five patients were cultured in 2D and 3D.
- Cells were treated with rhPDGF-BB.
- Apoptosis, proliferation, morphology, and gene expression were analyzed using flow cytometry, MTT assays, histology, and RT-PCR.
Main Results:
- rhPDGF-BB significantly reduced apoptosis in IVD cells.
- rhPDGF-BB increased cell proliferation and matrix production.
- rhPDGF-BB maintained the expression of key extracellular matrix genes.
Conclusions:
- rhPDGF-BB inhibits apoptosis and promotes anabolic activity in human IVD cells.
- rhPDGF-BB may serve as a future biologic therapy for early degenerative IVD disease.