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Gallic acid reduces cell viability, proliferation, invasion and angiogenesis in human cervical cancer cells
1Department of Health, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Abstract:
Gallic acid is a trihydroxybenzoic acid, also known as 3,4,5-trihydroxybenzoic acid, which is present in plants worldwide, including Chinese medicinal herbs. Gallic acid has been shown to have cytotoxic effects in certain cancer cells, without damaging normal cells. The objective of the present study was to determine whether gallic acid is able to inhibit human cervical cancer cell viability, proliferation and invasion and suppress cervical cancer cell-mediated angiogenesis. Treatment of HeLa and HTB-35 human cancer cells with gallic acid decreased cell viability in a dose-dependent manner. BrdU proliferation and tube formation assays indicated that gallic acid significantly decreased human cervical cancer cell proliferation and tube formation in human umbilical vein endothelial cells, respectively. Additionally, gallic acid decreased HeLa and HTB-35 cell invasion in vitro. Western blot analysis demonstrated that the expression of ADAM17, EGFR, p-Akt and p-Erk was suppressed by gallic acid in the HeLa and HTB-35 cell lines. These data indicate that the suppression of ADAM17 and the downregulation of the EGFR, Akt/p-Akt and Erk/p-Erk signaling pathways may contribute to the suppression of cancer progression by Gallic acid. Gallic acid may be a valuable candidate for the treatment of cervical cancer.
Insights
Gallic acid, a plant-derived compound, effectively inhibits human cervical cancer cell growth, invasion, and angiogenesis. It targets key signaling pathways, showing potential as a novel cervical cancer treatment.
Area of Science:
- Phytochemistry
- Oncology
- Cell Biology
Background:
- Gallic acid (3,4,5-trihydroxybenzoic acid) is a naturally occurring phenolic acid found globally in plants.
- Previous research indicates gallic acid exhibits selective cytotoxic effects on cancer cells, sparing normal cells.
Purpose of the Study:
- To investigate the anti-cancer effects of gallic acid on human cervical cancer cells.
- To determine its impact on cell viability, proliferation, invasion, and angiogenesis.
Main Methods:
- Human cervical cancer cell lines (HeLa and HTB-35) were treated with gallic acid.
- Assays included cell viability, BrdU proliferation, *in vitro* invasion, and human umbilical vein endothelial cell tube formation.
- Western blot analysis assessed protein expression (ADAM17, EGFR, p-Akt, p-Erk).
Main Results:
- Gallic acid reduced cervical cancer cell viability and proliferation in a dose-dependent manner.
- It significantly inhibited cell invasion and angiogenesis.
- Gallic acid suppressed ADAM17 expression and downregulated EGFR, Akt/p-Akt, and Erk/p-Erk signaling pathways.
Conclusions:
- Gallic acid demonstrates potent anti-cancer properties against human cervical cancer cells.
- Inhibition of ADAM17 and key signaling pathways (EGFR, Akt, Erk) contributes to its anti-cancer effects.
- Gallic acid represents a promising candidate for cervical cancer therapy.
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