Related Experiment Video
Updated: Jun 30, 2026

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
Published on: August 13, 2016
Kinesin-2 mediates apical endosome transport during epithelial lumen formation
Dongying Li1, E Wolfgang Kuehn2, Rytis Prekeris1
1Department of Cell and Developmental Biology; School of Medicine; Anschutz Medical Campus; University of Colorado Denver; Aurora, CO USA.
Abstract:
Apical lumen formation is a key step during epithelial morphogenesis of tubular organs. Appropriate transport and targeting of apical proteins to the apical membrane initiation site (AMIS) plays a crucial role in establishing a solitary, central lumen. FIP5, a Rab11-interacting protein, is an important regulator that directs apical endosome trafficking along microtubules toward the AMIS during cytokinesis. However, it is unknown which molecular motor(s) transports FIP5-positive apical endosomes during lumen initiation, and how this process is regulated. In this study, we demonstrate that the interaction of FIP5 with the microtubule motor, Kinesin-2, is required for the movement of FIP5-endosomes and delivery of these endosomes from centrosomes to the cleavage furrow during apical lumen initiation. Loss of Kinesin-2 disrupts targeting of apical proteins to the AMIS and results in multiple lumen formation in MDCK cysts. Our data provide more details to the molecular mechanism of FIP5-dependent apical trafficking during apical lumen formation.
More Related Videos
12:15In Vitro Polymerization of F-actin on Early Endosomes
Published on: August 28, 2017
12:15The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
Published on: October 3, 2017
Related Concept Videos
Microtubule Associated Motor Proteins
The Movement of Organelles and Vesicles
Anaphase A and B
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Mechanism of Lamellipodia Formation
Cytoskeletal Coordination in Cell Migration
Clathrin Coated Vesicles