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Updated: Apr 29, 2026

Peptide and Protein Quantification Using Automated Immuno-MALDI iMALDI
Published on: August 18, 2017
Spectral reproducibility and quantification of peptides in MALDI of samples prepared by micro-spotting
Yong Jin Bae1, Kyung Man Park, Sung Hee Ahn
1Department of Chemistry, Seoul National University, Seoul, 151-747, Korea.
Abstract:
Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.
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