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Chromate effects on red cells membranes.

D Beyersmann1, B Buttner

  • 1Department of Biology and Chemistry, University of Bremen, FRG.

Biological Trace Element Research
|July 1, 1989
PubMed
Summary

Chromate exposure increases human red blood cell (erythrocyte) resistivity and alters membrane breakdown voltage during electroporation. This study also observed shape changes and a shift in intracellular pH.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Previous studies investigated chromate modification of erythrocyte membrane proteins using kinetic and electrophoretic methods.
  • Chromate is a known environmental and industrial contaminant with potential biological effects.

Purpose of the Study:

  • To investigate the effects of chromate on human erythrocyte membrane properties and intracellular environment.
  • To elucidate the impact of chromate on electroporation parameters and cell morphology.

Main Methods:

  • Utilized Coulter Counter technology to measure intracellular resistivity and critical voltage for electroporation.
  • Observed changes in erythrocyte shape (echinocyte formation).
  • Monitored intracellular pH shifts induced by chromate exposure.

Main Results:

  • Chromate (10 mM) significantly increased intracellular resistivity of human erythrocytes.
  • Augmented the critical voltage required for membrane breakdown during electroporation.
  • Induced a slight echinocyte shape change and a shift towards higher intracellular pH values.

Conclusions:

  • Chromate exposure alters human erythrocyte membrane integrity and electrical properties.
  • Chromate affects cell morphology and intracellular pH, suggesting broader cellular impacts.
  • Electroporation parameters are sensitive indicators of chromate-induced cellular modifications.

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