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Membrane nanowaves in single and collective cell migration
Omar F Zouani1, Veronika Gocheva2, Marie-Christine Durrieu1
1Bioingénierie Tissulaire (BioTis), INSERM U1026, Université de Bordeaux, Bordeaux, France; Institut Européen de Chimie et Biologie (IECB), CNRS, UMR 5248, Université de Bordeaux I, Pessac, France.
Plos One
|May 22, 2014
Summary
This study characterizes 3D membrane waves in migrating cells using optical profiling. BMP-2 treatment enhances cell migration and wave propagation, with implications for skin tissue regeneration.
Area of Science:
- Cell Biology
- Biophysics
- Nanotechnology
Background:
- Cell migration is crucial for development and disease.
- Understanding cell membrane dynamics is key to motility.
- Previous studies lacked high-resolution 3D membrane wave characterization.
Purpose of the Study:
- To characterize three-dimensional (3D) membrane waves in migrating single and collective cells.
- To investigate the propagation and amplitude of these waves.
- To explore the effect of Bone Morphogenetic Protein-2 (BMP-2) on membrane wave dynamics and cell migration.
Main Methods:
- Wide-field optical profiling technique with nanometer resolution.
- Characterization of membrane wave propagation in single and collective cell migration.
- Analysis of wave amplitude and directionality under varying conditions (e.g., BMP-2 treatment).
Main Results:
- Existence of small (3-7 nm) and large (16-25 nm) 3D membrane waves.
- Single-cell migration shows waves up to 30 nm near the edge; BMP-2 increases amplitude (>80 nm) and unifies wave direction.
- Collective migration reveals attenuated waves in leader cells and poor transmission to followers; BMP-2 improves transmission and wave sharing between cells.
Conclusions:
- 3D membrane waves play a significant role in modulating single and collective cell motility.
- BMP-2 treatment enhances cell migration by altering membrane wave dynamics.
- Findings suggest potential therapeutic applications of BMPs in skin tissue regeneration.
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