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HSP70 desensitizes osteosarcoma cells to baicalein and protects cells from undergoing apoptosis

Lianghua Ding1, Shuanghua He, Xiaoliang Sun

  • 1Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, No 185 Juqian Street, Changzhou, 213003, China.

Apoptosis : an International Journal on Programmed Cell Death
|May 22, 2014
PubMed

Insights

Baicalein induces apoptosis in osteosarcoma cells through a mitochondrial pathway. Targeting heat shock protein 70 (HSP70) may overcome drug resistance in cancer therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Baicalein demonstrates broad-spectrum anti-cancer properties.
  • The effects of baicalein on osteosarcoma and its resistance mechanisms are not well understood.
  • Chemotherapy resistance is a significant challenge in treating osteosarcoma.

Purpose of the Study:

  • To investigate the anti-tumor effects of baicalein on human osteosarcoma cells.
  • To elucidate the molecular mechanisms underlying baicalein's action.
  • To identify potential mechanisms of drug resistance.

Main Methods:

  • Cell viability assays to assess baicalein's effect.
  • Apoptosis assays to detect programmed cell death.
  • Western blotting to analyze protein expression (HSP70, caspases, PI3K/AKT, MAPK/ERK pathways).

Main Results:

  • Baicalein induced apoptosis in osteosarcoma cells via mitochondrial pathways (caspase-dependent and independent).
  • Baicalein upregulated heat shock protein 70 (HSP70) expression, conferring partial resistance to apoptosis.
  • HSP70 activation of PI3K/AKT and MAPK/ERK pathways reduced osteosarcoma cell sensitivity to baicalein.

Conclusions:

  • Baicalein exhibits anti-osteosarcoma activity by inducing apoptosis.
  • HSP70 plays a crucial role in baicalein resistance through PI3K/AKT and MAPK/ERK signaling.
  • Targeting HSP70 in combination with baicalein could be a promising therapeutic strategy for osteosarcoma.

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